Capim marandu submetido à inoculação com bactérias diazotróficas associativas em latossolo vermelho de cerrado

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2013-12-09
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Santos, Carolina Silva Alves dos
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Universidade Federal de Mato Grosso
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Due to the quest for ways more viable and economical of supply nitrogen for the forage grasses, inoculation with associative endophytic diazotrophic bacteria is a potential alternative, because it contributes positively to the nutrition of plants, reducing the use of nitrogen fertilizers and will benefiting thus the adoption of this technology in the agricultural environment. The objective of this study was to evaluate nutrition, development and production of grass marandu submitted to the inoculation with diazotrophic bacteria in Oxisol of Cerrado. The experiment was conducted in a greenhouse at the Federal University of Mato Grosso, Campus of Rondonópolis, from December 2012 to March 2013, with a completely randomized design, composed by six treatments (three strains belonging to the collection of crops, similar to the Azospirillum amazonense, Herbaspirillum spp., and Burkholderia spp., respectively, and one commercial inoculant (formed by the composition of the strains ABV-5 and ABV-6 Azospirillum brasilense) completes fertilization (N, P, K, at doses of 100, 200 and 150 mg dm-3 respectively), control (no nitrogen and without inoculation) and five replications. Each parcel was composed per a vase of 8 dm3, totaling 30 parcels. The sowing was carried with fifteen seeds per pot, leaving themselves posteriorly five plants per pot. The inoculation was made by mean of the insertion of an aliquot of 3 mL of bacterial broth containing 108 cells ml-1 in the soil near the root area of each plant. Were perfomed three cuts in shoot of the plants at intervals of 30 days. After each cut, was taken the re-inoculation of the bacteria, so as to the nitrogen and potassium fertilization. The variables analyzed were plant height, number of leaves and tillers, dry mass of leaves and tillers, chlorophyll content Falker, pH of the soil, relation leaf/stem, relation shoot/root and concentration of nitrogen in the shoots and roots and concentration of crude protein. The results weresubmitted to analysis of variance and for separation of averages was used Tukey test at 5% probability of error. Significant effects were isolated and significant interaction between inoculation and cuts for most variables, with the bacteria that stood out were similar to strains of Burkholderia spp, Amazon Azospirillum and Herbaspirillum spp bacteria. with higher average dry mass of shoots (26.6, 22.05 and 22.75) compared to the control treatment.Therefore the inoculation of Brachiaria brizantha cv. Marandu with diazotrophic bacteria can contribute positively in their nutrition, production and development suggesting that the use of inoculants may be promising in reducing of the application of nitrogen fertilizers employed on forage grasses.
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SANTOS, Carolina Silva Alves dos. Capim marandu submetido à inoculação com bactérias diazotróficas associativas em latossolo vermelho de cerrado. 2013. 67 f. Dissertação (Mestrado em Engenharia Agrícola) - Universidade Federal de Mato Grosso, Instituto de Ciências Agrárias e Tecnológicas, Rondonópolis, 2013.
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